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Image Search Results
Journal: Regenerative Therapy
Article Title: Secreted matrix metalloproteinase-14 is a predictor for antifibrotic effect of IC-2-engineered mesenchymal stem cell sheets on liver fibrosis in mice
doi: 10.1016/j.reth.2021.08.004
Figure Lengend Snippet: Characterization of cell surface markers of MSCs by flow cytometry. (a) Representative histogram of the FACS of cell surface marker expression on BM-MSCs used in the present study. BM-MSCs from Lonza 22, Lonza 26, and Lonza 30 at passage 3 were labeled with FITC-coupled antibodies against CD34, CD45, CD90, CD105, and HLA-DR. Shaded areas indicated staining with antibodies against the indicat antigens, and solid lines indicate background fluorescence stained with isotype control. (b) Grouped bar chart showing quantitation of cell surface markers on each lot of three donors-derived BM-MSCs. The data are presented as mean ± SD from three independent FACS analyses. (c) The growth curve of the ten lots of BM-MSCs from L21 (Lonza 21) to L30 (Lonza 30). Cell culture was started at a cell density of 5.0 × 10 3 to 1.0 × 10 4 cells/cm 2 (P0) and passaged every 3–4 days to P1, P2, and P3.
Article Snippet: The data are presented as mean ± SD from three independent FACS analyses. (c) The growth curve of the ten lots of BM-MSCs from
Techniques: Flow Cytometry, Marker, Expressing, Labeling, Staining, Fluorescence, Control, Quantitation Assay, Derivative Assay, Cell Culture